[ES] Nuestro objetivo fue investigar la incidencia de la resistencia a antibióticos y biocidas en bacterias de origen alimentario. Para ello, hemos analizado mediante procedimientos dependientes e independientes de cultivo dicha resistencia a lo largo de la cadena de producción de la carne, así mismo hemos evaluado fenotípicamente y genotípicamente la resistencia en dos grupos microbianos patógenos tales como Pseudomonas sp. y Enterococcus sp. Nuestros resultados indicaron una alta resistencia a antibóticos de uso común en la clínica y biocidas frecuentemente usados en la industria alimentaria, así mismo hemos revelado los mecanismos moleculares implicados en dicha resistencia (genes específicos y bombas de exporte). Además, mediante estudios estadísticos, hemos determinado la interconexión entre ambos antimicrobianos la cual se ve reflejada por una resistencia cruzada entre algunos biocidas y antibióticos sugiriendo así que tipo de medidas a adoptar para evitar la diseminación de genes de resistencia a lo largo de la cadena alimentaria.

[EN] Our objective was to investigate the incidence of antibiotic and biocide resistance in food-borne bacteria. For this, such resistance was analyzed by culture dependent and independent methods throughout the chain of meat production, also phenotypic and genotypic resistance was evaluated in both pathogens microbial groups such as Pseudomonas sp. and Enterococcus sp. Our results indicated a high resistance to antibiotics commonly used in clinical treatments and to biocides frequently used in the food industry, also we have revealed the molecular mechanisms involved in this resistance (specific genes and efflux pumps) . In addition, we have determined by means of statistical studies the interconnection between both antimicrobials which was reflected by a cross-resistance between sorne biocides and antibiotics thus suggesting that such measures to be taken to prevent the spread of resistance genes throughout the food chain.

Submitted by Ana Baltar Gómez (aibaltar@ujaen.es) on 2016-03-08T13:33:54Z No. of bitstreams: 2 9788484399568.pdf: 11738790 bytes, checksum: dbd8817611c15be87d4f8841219df4ef (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)

Approved for entry into archive by Ana Baltar Gómez (aibaltar@ujaen.es) on 2016-03-08T13:34:07Z (GMT) No. of bitstreams: 2 9788484399568.pdf: 11738790 bytes, checksum: dbd8817611c15be87d4f8841219df4ef (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)

Made available in DSpace on 2016-03-08T13:34:07Z (GMT). No. of bitstreams: 2 9788484399568.pdf: 11738790 bytes, checksum: dbd8817611c15be87d4f8841219df4ef (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 206

Resistencia

Antibióticos

Biocidas

Genes de resistencia

Bacterias

Matadero

Alimentos fermentados tradicionales

Resistance

Antibiotics

Biocides

Resistence genes

Slaughterhouse

Tradictional fermented foods